SPIM-OPT, high lateral resolution with the best z resolution in 3D fluorescence imaging Light Sheet Fluorescence Microscopy (LSFM) or Selective Plane Imaging Microscopy (SPIM) are 3D imaging techniques that are becoming increasingly popular because they achieve excellent resolution at high penetration depths, while being minimally invasive. The lateral resolution of SPIM is comparable to that of a standard (epi) fluorescence microscope, while axial resolution is lower than the lateral (about a factor of 4). Optical Projection Tomography (OPT) can assume two primary modes - transmission and emission. In transmission mode, light traverses an optically cleared sample, providing general structural information. In emission mode, fluorescent labels in the sample are excited to produce specific functional information. OPT provides higher axial resolution than LSFM or SPIM, but lower lateral resolution. Our patented SPOT technology (Statistical Projection Optical Tomography) is a combination of SPIM and OPT, taking advantage of both technologies best features to produce high resolution in every dimension. [/lazy_load_box] Sample structural information add-on QLS-scope includes the possibility to perform an OPT measurement in transmission mode, where white light is passed through an optically cleared sample, and registered at 180o from the system camera. In this way one can obtain structural information about the sample of interest and can correlate this image with the Light Sheet Fluorescence Microscopy image that contains specific information about the sample.
Moderate demand for data storage space In Light Sheet Fluorescence Microscopy it is rather common to use a higher NA objective in order to achieve better lateral resolution. This results in a smaller FOV so, for large cleared samples, it is necessary to stitch several fields of view to reconstruct the whole sample. The final stitching file is often rather large, resulting in an increasing demand for data storage capacity. Our patented SPOT (SPIM-OPT) technology summarizes the stitched fields of view (imaged with a high NA objective) in just 500 images from different angles, independently of the size of the picture. All this procedure is done automatically and the amount of images to be stored are always around 500. This decreases a lot the demand for data storage. What to look for in a Light Sheet Microscope
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