High speed acquisition of big cleared samples

High speed acquisition of big cleared samples

Moving the Light Sheet instead of the sample, in combination with a high speed camera, permits the user to perform a SPIM measurement of a big cleared sample in a very short period of time. Each 2D image takes 7 ms to be registered by the camera. A big sample of 1cm wide, would need around 1000 z scans to be scanned completely (with a 10 micron z resolution). That means that it will take something more than one minute for the measurement to be finished. That is the highest speed of measurement ever, for such a big sample. Except the increased throughput of the system, the high speed of measurement offers protection from the sample photobleaching. A small explanation on how the QLS-scope performs SPIM measurements can be seen in the video below: